Ultrasensitive Detection of Chemical Substances
Quarterly rept. 1 Jun-31 Aug 1985
NEW MEXICO UNIV ALBUQUERQUE SCHOOL OF MEDICINE
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In this reporting period we have continued to characterize monoclonal antibodies against ADhE-DFP. We have focused attention three monoclonals of interest. These are the V-16 antibody described in our previous report and two additional antibodies designated V-18 and D11B. The V-16 antibody demonstrates higher activity against AChE-DFP conjugate than against AChE alone. The V-18 and D11B antibodies showed higher activity against AChE than against the conjugate. In addition, the D11B antibody blocks AChE enzymatic activity in the Elman assay. Further study showed that these antibodies exhibit a different response to AChE-DFP conjugates on the basis that the AChE ages or loses and isopropoxy residue. The resulting phosphate diester has a charge, at normal pH, on oxygen.