Accession Number:

ADA159796

Title:

Comparison of the Action of Types A and F Botulinum Toxin at the Rat Neuromuscular Junction,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

1985-01-01

Pagination or Media Count:

7.0

Abstract:

Blockade of neuromuscular transmission was produced in the lower hind limb of the rat by local injection of either thpe A or type F botulinum toxin BoTx. At 1,3,7 and 10 days after injection, the extensor digitorum longus edl nerve-muscle preparation was excised and analyzed for alterations in muscle mechanical properties or spontaneous and nerve stimulus-evoked quantal transmitter release. Muscles receiving type A toxin were paralyzed up to and including 7 days after injection. Muscles treated with type F toxin, although completely paralyzed at 1 and 3 days after injection, twitched in response to nerve stimulation by 7 days. Both toxins induced a marked decrease in the frequency of miniature end-plate potentials, but type A did so to a greater extent. Between 1 and 3 days after toxin injection nerve impulse-evoked transmitter release was reduced in both type A- and type F- treated muscles. Evoked release was temperature sensitive in type A-treated muscles but not in those treated with type F.

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE