Histologic Changes Caused by Application of Lewisite Analogs to Mouse Skin and Human Skin Xenografts
Final rept. 1 May 1983-30 Nov 1984
LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA
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Phenyldichloroarsine PDA, a vesicating analog of lewisite, was applied in an ethanol carrier to human skin xenografts on nude mice and directly to the ungrafted nude mouse skin. Controls areas received ethanol. Under light microscopy, we observed the following changes in PDA-treated human skin grafts 1 epidermal cellular nuclear degeneration apparent by 4 hr with increasing severity through 48 hr 2 loss of epidermal cytoplasmic basophilia apparent by 4 hr, maximum within 12 hr 3 epidermal cytoplasmic vacuolization vacuoles appeared within 4 hr and increased in size through 24 hr 4 cleft formation within the basement membrane zone apparent by 12 hr, increasing in severity through 24 hr 5 inflammation polymorphonuclear leukocyte PMN infiltration , apparent by 4 hr and increasing through 48 hr. The following additional lewisite analogs were applied in ethanol carriers to nude mouse skin phenylarsine oxide, phenydiiodoarsine, trans chlorovinylarsine oxide and trans chlorovinyldiiodide. The lesions caused by these analogs were reproducible and histologically indistinguishable from that cause by exposure to PDA. The identities of the molecular lesions and the locations of the arsenical- sensitive sites are unknown.