The Characteristics of Binding of Human Recombinant Interferon-Gamma to Its Receptor on Human Monocytes and Human Monocyte-Like Cell Lines,
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
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Interferon-gamma IFN-gamma induced many effector functions in macrophages, presumably through binding to cell surface receptors. Such receptors have been documented in murine macrophages. This report demonstrated that IFN-gamma interacts with a specific receptor on human monocytes and monocyte-like cell lines, U937 and HL60. Recombinant IFN-gamma rIFN-gamma was radioiodinated by using Bolton-Hunter re-agent to high specific activity. I- RIFN-gamma bound in a specific and saturable manner. Saturation of binding sites occurred at 10 to the minus 9th power M 300 Uml there were 4000 specific binding sites per cell bor both monocytes and the cell lines. Purified lymphocyte-derived IFN-gamma as well as rIFN-gamma competed for binding sites with I-rIFN-gamma. There was no inhibition of binding of the I-rIFN-gamma by RIFN-alpha or rIFN-beta.
- Medicine and Medical Research