Role of Protein Phosphorylation in Regulation of Bioreactivity.
Interim rept. 1 Mar 84-28 Feb 85,
ROCKEFELLER UNIV NEW YORK
Pagination or Media Count:
The time courses of synthesis of three neuronal cell-type specific phosphoproteins, Synapsin I, Synapsin III and G-Substrate, were determined in developing rat brains. The appearance of these proteins was concurrent with synapse formation. Two partial cDNA clones for Synapsin I were isolated from a rat brain cDNA expression library and the nucleotided sequences were determined. The mRNA for these clones was shown to be brain specific. Estimates for the sizes of the potential mRNAs for Synapsin I and Synapsin III were determined. A second rat brain cDNA expression library was generated for screening for synapsin III clones. A monoclonal antibody specific for synapsin III was identified for screening this library. A bovine caudate cDNA and rabbit cerebellar cDNA library were generated for obtaining, respectively, DARPP-32 and G-Substrate cDNA clones. Oligonucleotide probes specific for DARPP-32 and G-Substrate were synthesized for screening these libraries. Originator supplied keywords include Oligonucleotide Monoclonal Synapsin I CalciumCalmodulin.