Radiometric Methods for Rapid Diagnosis of Viral Infection.
JOHNS HOPKINS UNIV BALTIMORE MD
Pagination or Media Count:
Two radiometric techniques were developed for detecting the presence of herpes simplex virus type 1 in stationary monolayers of the diploid cell line WI-38. The time of detection was compared to that obtained from visual examination for cytopathic effects in the same cell line. Glucose-1-14 oxidation and DNA synthesis of infected and uninfected cells were determined by 14CO2 production measured by an ionization chamber, and 3H-thymidine incorporation measured by scintillation counting, respectively. Infected cells showed a 23 to 26 reduction in glucose-1-14C oxidation and 355 to 498 increase in DNA synthesis four to six hours postinfection as compared to uninfected control cells. These changes in cellular metabolism were observed 14 hours before visible signs of cytopathic effects. The increase in DNA synthesis was completely inhibited by viral neutralization with herpes simplex antiserum. Increased DNA synthesis was observed five hours postinfection with 10,000 to 1,000,001 TCID50 units of virus. The radiometric methods for the detection of viral effect on cellular metabolism are simple, fast, and objective. Author
- Medicine and Medical Research