Small X-Ray Diffraction of Immunoglobulin-Membrane Complexes.
Final technical rept. 15 Apr 81-14 Oct 84,
STANFORD UNIV CALIF W W HANSEN LABS OF PHYSICS
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In the first part of this research, specially designed hapten-phospholipid molecules were synthesized to enable the binding of immunoglobulins to artificial phospholipid membranes. In subsequent work, using these immunoglobulin-membrane complexes, electron microscopy was used to reveal the detailed crystalline structure of a two dimensional 2D immunoglobulin crystal attached to an electron microscope grid. These studies enabled the discovery of a new conformational arrangement of crystals formed from intact immunoglobulin molecules. In a third part of the study, monolayers of protein-membrane complex in this case the purple membrane from halobacterium halobium were deposited on a single crystal silicon substrate. Using the technique of grazing incidence X-ray diffraction, diffraction patterns from a single monolayer of protein-lipid complex were obtained for the first time.