The Evaluation of Qualitative and Quantitative Procedures in the Detection of IgA - Deficient Donors.
AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH
Pagination or Media Count:
IgA-deficient blood products are required to avoid potentially fatal anaphylactic transfusion reactions in patients who are IgA-deficient and have formed anti-IgA antibodies. Red blood cells for these patients may be provided through autologous transfusion or extensive washing of banked or frozen units. However, when other blood components such as fresh frozen plasma or cryoprecipitates are needed, the use of a rare donor file of IgA-deficient individuals is required. The frequency of IgA deficiency has been reported to vary between 1300 to 13000 in normal, healthy donor populations. The large number of samples to be tested to create a donor file of IgA-deficient individuals requires a sensitive, accurate and cost effective screening methodology. This study was performed to evaluate qualitative immunodiffusion and quantitative hemagglutination-inhibition HI procedures for use in the identification of IgA-deficient individuals. The sensitivity and accuracy of radial immunodiffusion and double diffusion and HI were evaluated with previously identified IgA-deficient samples.
- Medicine and Medical Research