Recognition of Serogroup A Neisseria meningitidis Serotype Antigens by Human Antisera,
CALIFORNIA UNIV OAKLAND NAVAL BIOSCIENCES LAB
Pagination or Media Count:
The antigens of Neisseria meningitidis serogroup A which were recognized by human antisera were identified by Western blot and enzyme-linked immunosorbent assay techniques. The components of six prototype strains used for serotyping serogroup A meningococci were resolved by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and then transferred to nitrocellulose for immunoperoxidase staining with sera collected from 10 acute-phase and 14 convalescent-phase patients. Six acute-phase sera detected six major antigens having apparent molecular weights between 14,000 and 82,000. In addition to recognizing these antigens, the convalescent-phase sera detected a protease- sensitive antigen with an apparent molecular weight of 20,000 for one strain and 27,000 for five strains, lipopolysacchride, and the heat-modifiable proteins. The sera recognized lipopolysaccharide in a serotype-specific manner, whereas their reactions with the heat-modifiable protein were not serotype specific. Convalescent-phase sera recognized components from eight meningococcal serogroups. The concentrations of immunoglobulin G directed to capsular polysaccharide were determined by enzyme-linked immunosorbent assay seven acute-phase sera had less than 0.39 micrograms of antibody per ml, whereas the average concentration in convalescent-phase sera was 3.22 microgramsml and the range was 0.40 to 7.50 microgramsml.
- Medicine and Medical Research