Accession Number:

ADA154415

Title:

Molecular Toxicology of Chromatin: The Role of Poly(ADP-Ribose) in Gene Control.

Descriptive Note:

Annual progress rept. Oct 83-31 Dec 84,

Corporate Author:

CALIFORNIA UNIV SAN FRANCISCO

Personal Author(s):

• Kun,E.

Report Date:

1985-02-01

Pagination or Media Count:

137.0

Abstract:

This report pertains to the following research projects Chemical and macromolecular structure of polyADP-ribose1. HPLC-isolation of polyADP-ribose.. 2. Fractionation, size analysis branching of poly ADP-ribose by HPLC and chemical analysis of subunits. 3. Mathematical model of polymerization of ADP-ribose. II. Biosystems. III. Molecular studies on purified polyADP-ribosylation in cellular and subcellular systems. III. Molecular studies on purified polyADP-ribose polymerase system. 1. DNA-association of benzamide. 2. The role of lysine residues in the catalysis and DNA binding of polyADP-ribosepolymerase. IV. Cell transformation and poly ADP-ribosylation. 1. Inhibition of carcinogen initiated transformation. 2. Ultraviolet light induced transformation and its inhibition.

Descriptors:

• *GENES
• *TOXICOLOGY
• *ADENOSINE PHOSPHATES
• *CHROMATIN
• *RIBOSE
• MATHEMATICAL MODELS
• ULTRAVIOLET RADIATION
• MOLECULES
• POLYMERIZATION
• DEOXYRIBONUCLEIC ACIDS
• LIQUID CHROMATOGRAPHY
• MACROMOLECULES
• MOLECULAR STRUCTURE
• CATALYSIS
• AMIDES
• INHIBITION
• CELLS(BIOLOGY)
• AMINO ACIDS
• TRANSFORMATIONS
• FRACTIONATION

Subject Categories:

• Biochemistry
• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE