Accession Number:

ADA151986

Title:

A Study of the 5S Ribosomal RNAs of the Vibrionaceae

Descriptive Note:

Doctoral thesis

Corporate Author:

MARYLAND UNIV COLLEGE PARK DEPT OF MICROBIOLOGY

Personal Author(s):

• MacDonell, M. T.

Report Date:

1984-01-01

Pagination or Media Count:

149.0

Abstract:

The sequence of the 120 nucleotide bases of 5S ribosomal RNA 5SrRNA has been determined for each of 23 eubacterial strains classified as species of the family Vibrionaceae. The methods employed included the resolution of limited enzymatic digests of end-labeled RNAs by high voltage polyacrylamide gel electrophoresis, as well as thin-layer chromatography. Several of the 5S rRNAs, for which the primary structures were determined, were also subjected to secondary structure analysis, employing nuclease S1, which hydrolyzes regions of nucleic acids not participating in the formation of helices, followed by resolution of the partial digests on thin sequencing gels. Sequence data obtained from this study were compiled and analyzed, using statistical methods and group-specific signature analyses, for the purpose of constructing a phylogenetic taxonomy of the Vibrionaceae. Dot matrix maps were generated for 5S rRNA sequences determined in this study, in order to analyze the occurrence, extent, and complexity of palindromic and repeated base sequences.

Descriptors:

• *EUBACTERIALES
• *RIBONUCLEIC ACIDS
• *RIBONUCLEOTIDES
• *RIBOSOMES
• ELECTROPHORESIS
• MAPS
• SEQUENCES
• STATISTICAL PROCESSES
• STRUCTURAL ANALYSIS
• THESES
• THIN LAYER CHROMATOGRAPHY

Subject Categories:

• Biochemistry
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE