Accession Number:

ADA151720

Title:

Enzymatic Conversion of Red Cells for Transfusion.

Descriptive Note:

Annual rept. 1 Mar 84-28 Feb 85,

Corporate Author:

NEW YORK BLOOD CENTER N Y

Personal Author(s):

• Goldstein,J.

Report Date:

1985-03-01

Pagination or Media Count:

13.0

Abstract:

Our interest is in enzymatically converting type A and B erythrocytes to type O under conditions which render them useful in transfusion therapy. We are currently attempting such conversions of A cells using an alpha-N-acetylgalactosamindase A-zyme for chicken liver. This enzyme has been purified free of any detectable contaminating proteases, sialidase and other exoglycosidases with the the exception of alpha-galactosidase activity 8-10 of A-zyme which is believed to be intrinsic and not due to a contaminating enzyme. It can remove A antigenicity from the red cell surface and is considerably more effective in doing so than comparable preparations of A-zyme from the earth worm and sheep. Results are encouraging in that they suggest that A antigenic sites resistant to the action of chicken liver A-zyme are not sterically inaccessible but given the proper enzyme and buffer conditions they too can be removed. Originator-supplied keywords include Enzymatic conversion of type A cells to 0, Alpha-N-acetylgalactosaminidase or A-zyme, A-zyme treatment of type A cells.

Descriptors:

• *ENZYMES
• *CATALYSIS
• *ERYTHROCYTES
• *BLOOD GROUPS
• CONVERSION
• LIVER
• CHICKENS
• ANTIGENS
• BLOOD TRANSFUSION

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE