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Accession Number:
ADA151542
Title:
Identification, Synthesis, and Characterization of the Yolk Polypeptides of Plodia interpunctella,
Descriptive Note:
Corporate Author:
OREGON STATE UNIV CORVALLIS
Report Date:
1984-01-01
Pagination or Media Count:
13.0
Abstract:
The mature eggs of Plodia interpunctella were found to contain four major polypeptides. These yolk polypeptides YPs were found to have approximate molecular weights of 153,000 daltons YP1, 69,000 daltons YP2, 43,000 daltons YP3, and 33,000 daltons YP4 as determined by sodium dodecyl sulfate- polyacrylamide gel electrophoresis SDS-PAGE. In addition, we found YP1 was resolved by a 5 polyacrylamide gel into two separate polypeptides of 153,000 and 147,000 daltons. All of the YPs could be labeled in vivo or in vitro with 35S-methionine. Yolk peptide 1 and YP3 were synthesized by body fat of pharate adult and adult females and secreted into the hemolymph. Yolk peptide 2 and YP4 were synthesized and secreted into incubation medium by ovaries that contained vitellogenic cocytes, but these polypeptides were not found in the hemolymph. Fat bodies of males synthesized and secreted an immunoprecipitable polypeptide similar to YP3 as well as immunoprecipitable polypeptides larger than 200,000 daltons that had no counterparts in the oocytes. Peptide mapping by protease digestion showed each YP to be cleaved into unique fragments, suggesting that no precursor product relationship exits between the YPs.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
