Biochemistry of Trypanosomatidae of Importance in Africa.
Annual rept. 1 Jan-31 Dec 82,
MASSACHUSETTS UNIV AMHERST DEPT OF MICROBIOLOGY
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A comparison of the enzymes of pathogenic protozoa to those of man is of fundamental importance to the search for much needed chemotherapeutic agents. The enzymes involved in purine salvage are of particular interest because most pathogenic protozoa lack the ability to synthesize purines de novo and consequently are obligate salvagers of preformed purines. This project involves an investigation of purine and pyrimidine metabolism of Leishmania. Comparisons of their biochemistry will be made within the parasitic group and to their host cells. Basic information regarding metabolic capacities of these organisms will be obtained. Attention will be given to the mechanisms by which these organisms absorb nutrients from their environment. These mechanisms involve enzymes excreted into their surroundings, enzymes located on the cell surface, and enzymes located within the cell. During this year uptake capabilities of Leishmania donovani Khartcum strain-drug sensitive visceral leishmaniasis and Leishmania braziliensis panamensis WR 227 were investigated. In L. donovani WR 130 it was found that N6 methylamino-purine inhibited uptake of hypoxanthine, guanine and to a lesser extent adenine. 6-Methylaminopurine 9-ribofuranoside inhibited uptake of adenosine and to a lesser extent guanosine.
- Medicine and Medical Research