Cellular Effects of Perfluorinated Fatty Acids (PFDA).
Annual rept. 1 Jan 83-31 Dec 83,
CALIFORNIA UNIV IRVINE
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This is a proposal to investigate the effects of perfluorinated decanoic acid PFDA on the cell surface of liver cells and tissue. The major method of approach is by using fluorescence photobleaching and scanning electron microscopy of the cell surface. The results of this first year are summarized as follows a differentiated liver tissue culture cells in vitro do have the membrane fluidity affected by PFDA whereas undifferentiated, non-liver cells are not affected b the fluidity changes are transient with recovery by 24-48 hrs. c the effect on membrane mobility is dose dependent d the effect on mobility occurs within 24 hours of exposure without further increase with time of exposure e scanning EM demonstrates no gross structural abnormality of the surface as a result of the non-toxic levels of PFDA.