Ultrasensitive Detection of Chemical Substances
Quarterly rept. 1 Jun-31 Aug 1984
NEW MEXICO UNIV ALBUQUERQUE SCHOOL OF MEDICINE
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We have continued in our efforts to quantitate the relative binding constants of our monoclonal antibodies and in reducing noise factors. We were able to measure the intrinsic affinity constant of protein A purified antibody specific for AChE-DFP conjugate. Since affinity constants reflect the dissociation and association constant, and association constants will be similar for antibodies to a particular antigen, we can test our monoclonal antibodies for their relative strength of binding using affinity constant calculations. Thus, for the time being, we see no essential need to calculate dissociation constants directly.
- Medicine and Medical Research