Effect of Perfluorodecanoic Acid on the Efflux of 2-Aminopurine from L5178Y Cells
Annual research rept. 1 Aug 1983-31 Jul 1984
TENNESSEE UNIV MEMORIAL RESEARCH CENTERKNOXVILLE DEPT OF MEDICAL BIOLOGY
Pagination or Media Count:
Transporters in the cell membrane of animal cells are susceptible to the action of toxic environmental agents. L5178Y mouse leukemia cells were treated with 150 microgramsml perfluorodecanoic acid PFDA, a toxicant, in growth medium for 24 hr at 30 C. The PFDA-treated cells were transferred to fresh growth medium to demonstrate recovery of the capacity to transport a fluorescent purine, 2-aminopurine AP. The membrane flux of AP was estimated with a high-speed kinetic assay. The cells were preloaded with 100 micrometers AP and rinsed twice in a cold buffer. The preloaded cells were resuspended in buffer at 21 C and AP flux was estimated continuously from the fluorescence emission of AP at 370 nm. At the beginning of the recovery period, AP efflux from PFDA-treated cells was markedly inhibited in comparison with the untreated controls. After 2 days at 37 C, the count of PFDA-treated cells doubled and the initial rate of AP efflux returned to approximately 60 of the controls. After 6 days at 30 C, the cell count increased 2.7-fold and AP efflux returned to approximately 60 of the controls. The results of experiments with untreated control cells show that the initial rate of AP efflux is proportional to the intracellular AP concentration.