Characterization of Antibody-Dependent Killing of Trypanosomes by Macrophages.
Annual summary rept. 15 Jan 82-14 Jan 83,
ALBERT EINSTEIN COLL OF MEDICINE BRONX NY
Pagination or Media Count:
In the presence of antibody, macrophages bind trypanosomes in vitro. The cellular and humoral components of this assay have been studied and defined. In general, such antibody must be specific for the trypanosome variant. However, WRATat-14 and WRATat-15 variants, obtained after vector transmission of WRATat-1.1, mediated of WRATat-1 trypanosomes to macrophages. Using a large panel of anti-WRATat-1.1 specific monoclonal antibodies, a close correlation was found between the ability to mediate macrophage binding in vitro, and in vivo trypanosome neutralization. The relative efficacy of various immunoglobulin subclasses was analyzed. Monoclonal antibodies of all subclasses were able to mediate trypanosome binding by macrophages. However, when polyvalent anti-WRATat-300 Wellcome serum from a rabbit infected 4 weeks was fractionated into isotype specific-immunoglobulins, only the cold-precipitable portion of IgM was functional. The role of complement was also analyzed. It was confirmed that complement enhances macrophages binding of trypanosomes in the presence of limiting dilutions of antiwerum. There was no species specificity in the action of complement since dog, human, guinea pig and mouse sera could be used as complement sources.
- Medicine and Medical Research