Tissue Culture and in Vivo Modelling of Corneal Opacification and Ocular Injuries Induced by Millimeter Waves.
Annual rept. 1 Oct 80-30 May 81,
UNIVERSITY OF WESTERN ONTARIO LONDON DEPT OF BIOCHEMISTRY
Pagination or Media Count:
Conditions were established for in vitro culturing of rabbit corneas in preparation for experiments in which irradiation with millimeter waves would be performed in vitro. Alpha medium was found to be appropriate for culturing corneal epithelial cells and whole corneas for short periods up to one week. Incubation of corneas at elevated temperatures from 39-50 C resulted in progressive disruption of normal cell structure, beginning at low temperatures 39, 42 with apparent swelling of cells, loss of normal structure of microville, and depressed rather than raised cell junctions, and at higher temperatures 43 C, 50 C to loss of microvilli, curling of cells and deepithelialization.