Determination of the Toxicity to Aquatic Organisms of HMX and Related Wastewater Constituents. Part 2. The Acute and Chronic Toxicity of Acetone, Dimethyl Formamide and Triethylene Glycol to Daphnia Magna (Straus).
Final rept. 1 Feb 80-31 Dec 82,
EG AND G BIONOMICS WAREHAM MASS
Pagination or Media Count:
Acute and chronic toxicity tests were performed with three organic solvents commonly used to facilitate the solubilization of lipophilic compounds during aquatic toxicity tests with Daphnia magna Straus. The 48-hour LC50 values and corresponding 95 confidence intervals were acetone, 3900031000-53000 microlitersL dimethyl formamide, 1300010000-16000 microlitersL triethylene glycol, 3500028000-46000 microlitersL. Maximum acceptable toxicant concentrations determined during the chronic toxicity tests were acetone, 14002800 microlitersL dimethyl formamide, 12002500 microlitersL triethylene glycol, 550011000 microlitersL. Triethylene glycol was the least chronically toxic solvent and is recommended as the primary choice when selecting a carrier solvent during aquatic toxicity tests. All three solvents were sufficiently low in toxicity to suggest that the recommended usage limits 500 microlitersL during acute toxicity tests, 100 microlitersL during long-term toxicity tests are adequate for the prevention of solvent-related toxicity to D. magna.