The Effects of EDTA (Ethylenediaminetetraacetic Acid) and Sonication on the Disaggregation of Oral Bacteria,
ARMY INST OF DENTAL RESEARCH WASHINGTON DC
Pagination or Media Count:
The dispersive effects of ethylenediaminetetraacetic acid EDTA and ultrasound on bacterial aggregates in an anaerobic environment were studied. Polled supragingival plaque samples were placed into reduced transport fluid RTF containing 1 mmol, 10 mmol, 100 mmol EDTA, or EDTA-free RTF for dispersion, and colony forming units cfu were determined after culture at 37 C for 72 hours. Mean colony forming units were increased 42.7 and 54.4 respectively in 1 mmol and 10 mmol concentrations of EDTA and decreased when plaque was dispersed in 100 mmol concentrations of EDTA. Fusobacterium nucleatum was cultured in broth medium, placed into RTF containing no chelating agent, and sonicated for either 0, 5, 15, or 30 seconds. Sonication at the lowest power setting produced a statistically significant p.01 increase in CFU after 5 seconds. This trend did not continue when time of sonication was increased. The effect of sonication at medium or high power settings was variable, and factors affecting variability are discussed. EDTA and sonication may enhance the quantitation of oral microorganisms.
- Medicine and Medical Research