Effects of Radiofrequency Radiation on Differentiation of Erythroleukemic Cells.
Final rept. 2 Sep 82-1 Feb 84,
Pagination or Media Count:
A dose-response study was conducted to determine if chemically induced erythroid differentiation of murine erythroleukemic MEL cells is affected by continuous-wave radiofrequency RF radiation. RF exposures were at 1180 MHz in a specially constructed anechoic chamber equipped with a constant-temperature-air circulator designed to maintain the cell at 37.4 C. Experiments were performed at incident power densities of 5.5, 11, and 22 mWsq cm, corresponding to SAR levels of 18.5, 37, and 74 Wkg, respectively. Four replicate experiments were conducted at each power level with two irradiated and two control cultures included in each replicate. Cultures were initiated by suspension of MEL cells in growth medium containing the inducer hexamethylene biasacetamide, 3 mM, in 10-cm cellulose nitrate tubes. One day after addition of the inducer, the cultures were placed in the exposure chamber and irradiated for 48 hr. Cells were then counted, resuspended in normal growth medium no inducer, and incubation continued for 2 additional days. Number of cells undergoing erythroid differentiation was determined by staining with benzidine, a hemoglobin-specific reagent. Amount of hemoglobin present in lysates prepared from the differential cells was determined by a colorimetric procedure.