Effect of Hepatotoxic Nonadecafluorodecanoic Acid on Gene Expression in Rat Liver
Final rept. 15 Apr 1983-14 Jan 1984
OHIO STATE UNIV RESEARCH FOUNDATION COLUMBUS
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Nonadecafluorodecanoic acid NDFDA, a biological test model for similar compounds used to produce aqueous film-forming foams, is a potent hepatotoxin. since the compound has been shown to modify cellular membrane systems, the effect of NDFDA on membrane-dependent functions of genetic expression hs been investigated. Messenger RNA transport through nuclear pores in a cell-free system derived from rat liver, was significantly inhibited when the nucleic were derived from NDFDA-treated rats. The induction of the hepatic enzyme tyrosine aminotransferase by hydrocortisone and insulin was near normal. In contrast, pretreatment with NDFDA inhibited induction by glucagon, suggesting that some receptor or post-receptor step in the glucagon-mediated activation of the membrane-bound adenylate cyclase system was defective.
- Anatomy and Physiology