Use of Sperm Enzymes to Detect Genotoxic Agents.
Final rept. Feb 83-Feb 84,
WESTERN MICHIGAN UNIV KALAMAZOO DEPT OF BIOMEDICAL SCIENCES
Pagination or Media Count:
The sperm enzyme test SET system consists of a series of histochemical assays that can be used to assess the functionality of sperm. The objective of this research was to determine the usefulness of the SET system in evaluation of germ cell damage which might be caused by a variety of chemicals. Mice were treated with several known mutagenic or teratogenic agents, or chemicals toxic to the reproductive system two and six weeks before collecting sperm. Sperm was stripped from the vas deferens and examined for count, motility, and the sperm enzymes, acrosin, hyaluronidase, succinic dehydrogenase SDH, and alpha glycerolphosphate dehydrogenase aGDH activities. Groups of mice were treated through intraperitoneal i.p. or inhalation i.h. routes. Two weeks after treatment, the chemicals ethylnitrosourea ENU, ethyl methanesulfonate EMS, hydroxyurea HU, cigarette smoke condensate CSC, dimethyl methylphosphonate DMMP, perfluoro-n-decanoic acid PFDA, ethylene dibromide EDB, and benzene were found to be positive by the SET system. At six weeks, only ENU, HU and DMMP exposures caused enzyme changes e significantly different from controls, while only ENU and HU caused reduced testis weight, a conventional method of germ cell damage assessment. Two weeks after i.h. treatment, benzene and EDB were found to be positive for sperm enzyme changes, while at six weeks postexposure only DMMP was detected.