Accession Number:

ADA142671

Title:

Karnovsky-Osmium as a Primary Fixative for Electron Microscopy.

Descriptive Note:

Scientific rept.,

Corporate Author:

ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Personal Author(s):

• Buchanan,G. M.

Report Date:

1983-09-01

Pagination or Media Count:

5.0

Abstract:

A combined Karnovsky-osmium tetroxide primary fixative is proposed for transmission electron microscopy. This fixative, hypothetically, seems to overcome the drawbacks of sequential fixation techniques. Evaluation of this combined fixative reveals very acceptable fixation of hepatocytes, lymphocytes, and fibroblasts, as well as various organelles within these cells. Author

Descriptors:

• *ELECTRON MICROSCOPY
• COMPARISON
• SEQUENCES
• TRANSMITTANCE
• FIBROBLASTS
• LIVER
• MONOCYTES
• CELLS(BIOLOGY)
• LYMPHOCYTES
• TETROXIDES
• OSMIUM COMPOUNDS

Subject Categories:

• Optics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE