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Chemical Carcinogen (Hydrazine et al.) Induced Carcinogenesis of Human Diploid Fibroblasts in vitro.
Final rept. 1 Jul 80-30 Nov 83,
OHIO STATE UNIV RESEARCH FOUNDATION COLUMBUS
Pagination or Media Count:
The initiation of a carcinogenic insult appears to be optimally effective when the cells are insulted in early S phase of the cell cycle. The transformed phenotype has sarcoma associated determinants that are similar to the ectopic determinants found on human sarcoma-tumor tissue. Both these cell types, i.e. chemically transformed human fibroblasts and sarcoma tumor cells, exhibit cellular invasiveness, neoplastic potential and finite but extended lifespan in vitro. Using selection pressures for culturing of human normal cells in culture followed by insulting the DNA we can isolate cells from the transformed phenotype, from cells that will respond to the insult as a toxic insult. The carcinogen-specific DNA adducts formed under these conditions are qualitatively and quantitatively similar.
APPROVED FOR PUBLIC RELEASE