Stability and Function of Granulocytes Isolated by Counterflow Centrifugation and Discontinuous Density Gradients of Ficoll-Hypaque.
Final technical rept. 8 Jan 83-7 Jan 84,
CENTER FOR BLOOD RESEARCH BOSTON MA
Pagination or Media Count:
Granulocytes isolated by counterflow centrifugation elutriation CCE were compared with those isolated by dextran sedimentation, centrifugation with Ficoll-Hypaque, and lysis of residual red cells SFWL. They were also compared with granulocytes isolated on discontinuous density gradients using two densities Ficoll-Hypaque DFDG. The cells were stored at 4 C for 72 hours and aliquots measured for phagocytic indices particle ingestion, membrane integrity fluorescein-diacetate-ethidium bromide volume electronic sizing and membrane function superoxide anion. CCE and SFWL prepared granulocytes were more stable than DFDG cells, The SFWL method is preferred over CCE because of its simplicity and the high quality and purity of the isolated granulocytes.
- Medicine and Medical Research