Responses of Cholinergic and Noncholinergic Renshaw Cell Receptors After Acute and Chronic Exposure to Anticholinesterases.
Annual summary rept. 15 Aug 80-14 May 81,
IOWA STATE UNIV AMES
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Investigation of cat lumbar 7 segment L-7 ventral horn cells motoneurones and interneurones-Renshaw cells in the presence of excess acetylcholine ACh are being studied as models of a central cholinergic transmitting synapse. Preliminary studies to investigate effects induced by chronic low dose exposure to diisopropylfluorophosphate DFP have begun with a two week exposure to diisopropylfuorophosphate DFP have begun with a two week exposure protocol with DFP doses of 0.1, 0.2, 0.5, 0.75, and 1.0 mgkgday s.c. Cats tolerate doses of 0.1 to 0.5 mgkgday s.c. with symptoms of increased defecation, moderate constriction of pupils slight ataxia and weight loss. The degree of severity increases with dose. Renshaw cell unit potentials RUP and their field potentials RFP are being studied to determine effects from exposure to DFP in doses according to the protocol above. In control animals, RFP respond similar to RUP during and subsequent to repetitive stimulation. Effects of drug treatment on the ventral horn neurones of L-7 are being studied with light microscopy LM and electron microscopy EM histochemistry.
- Anatomy and Physiology