Ultrasensitive Detection of Chemical Substances
Final rept. 31 Dec 1983-31 Jan 1984
NEW MEXICO UNIV ALBUQUERQUE SCHOOL OF MEDICINE
Pagination or Media Count:
The most important element in our scheme of detection is the ability to produce monoclonal antibodies, particularly to the determinate specific for the complex between the hapten and trapping agent. We have been quite successful in producing monoclonal antibodies. We have now five monoclonal antibodies against AChE-DFP conjugates. Another important element in developing an ultrasensitive assay is to produce signal packets with appropriate fluorescent signal characteristics and the ability to bind antibody. In addition, characteristics of low non-specific binding to solid substrates and the ability to link to a solid substrate with specific binding that is stable to various washing procedures are important considerations in selecting signal packets. We were very fortunate that covaspheres, a new product of the Covalent Technology Corporation, were being developed shortly before we began work. These beads have a very strong fluorescent signal and very little fading which gives excellent counting characteristics with a microscope at only 100 magnification.
- Medicine and Medical Research