An Investigation of the Memory Response of the Local Immune System to Shigella Antigens.
Annual progress rept. 1 Feb 81-31 Jan 82,
MICHIGAN UNIV ANN ARBOR DEPT OF PATHOLOGY
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Rabbits were immunized orally with 3 doses of live noninvasive, Shigella flexneri strain 2457-0. Sixty days after the third oral dose a chronically isolated ileal loop was created surgically in each rabbit. The local IgA and IgG anti-shigella responses in secretions and sera were followed with a sensitive enzyme-linked immunosorbent assay. A significant local IgA memory response was found in the secretions of the orally-primed rabbits. Parenteral immunization alone results in erratic and weak local IgA production. Further, with a dosage schedule was that achieved serum IgG activity to shigella antigens, parenteral immunization as not able to prime the rabbits for local, intestinal IgA memory response. In other studies, it was found that erythromycin interferred with development of IgA memory responses and adjuvant DEAE-dextran had no significant effect on the primary local IgA response to orally-administered Shigella flexneri. The effect of this proposed adjuvant on the IgA memory response remains to be investigated the present studies demonstrate that a local IgA memory response to Shigella flexneri can be elicited by oral priming with a live, noninvasive strain. Further, parenteral vaccination was ineffective in priming for a mucosal IgA memory response. Author
- Medicine and Medical Research