Dye Fluorescence Analysis from Bacterial Metabolism.
Technical rept. Dec 82-Mar 83,
CHEMICAL RESEARCH AND DEVELOPMENT CENTER ABERDEEN PROVING GROUND MD
Pagination or Media Count:
A method based upon induced fluorescence is proposed for the rapid detection and characterization of viable microorganisms. In this technique, a nonfluorescing dye is metabolized intracellularly by an organism through an enzyme-specific reaction. This produces a fluorescent product which when desorbed can be detected. The technique has been applied successfully to the microorganism Bacillus globigii through the enzyme lipase and the nonfluorescing dye diacetylfluorescein. By relating the effect of bacterial and substrate activity to the initial rate of fluorescence generation, standard curves have been obtained from which both age and bacterial concentration can be estimated.