The Use of Physarum for Testing of Toxicity/Mutagenicity
Final rept. 1 May 1982-30 Jun 1983
GOVERNORS STATE UNIV PARK FOREST SOUTH IL SCIENCE DIV
Pagination or Media Count:
Growing and differentiating cultures of the true slime mold, Physarum polycephalum, have been investigated for possible use in testing for toxicity of several fuels--hydrazine, ethanol and hydrocarbons. Testing protocols were based on several already in the literature. Effects on growth were studied via inhibition of microplasmodial growth Becker et al., Cancer Res. 23, 1910, 1963 and effects on differentiation by inhibition of or interference with plasmodial sporulation Sauer et al., Exptl, Cell Res. 57, 319 1969 and of amoebal flagellation Mir and Wright, Microbios. Lett. 5, 39, 1978. Effects on growth of microplasmodia were expressed as ED50 values, calculated from spectrophotometric measurements of while culture protein, DNA or pigment. ED50S protein data for hydrazine.2 HC1 and ethanol were 40 ugml and 1.5 vv, respectively. Short, straight chain, even numbered hydrocarbons C6 - C10 at 1 completely stopped microplasmodial growth. The C12 - C16 hydrocarbons at the same concentration did not inhibit growth and may have served as a carbon source, since they seemed to have a glucose-sparing effect.