Actions of Interferons on Macrophages.
Annual rept. no. 1, 1 Jun 83-31 May 84,
TRUDEAU INST INC SARANAC LAKE NY
Pagination or Media Count:
The successful immunization of rats with a partially purified IFN gamma produced by the closely related murine species. The generation of transspecies hybridomas through the fusion of rat immune B-cells to mouse myeloma P3U-1 cells. Screening the supernatants of hundreds of wells containing hybridomas from multiple fusion efforts, revealed that the medium from a single well addressed the ability to neutralize the antiviral activity of MuIFN gamma. From this well, a hybridoma secreting a rat anti-MuIFN gamma monoclonal antibody MAb was cloned. The hybridoma producing rat anti-MuIFN gamma MAb has proven stable and is being propagated both in vitro and as well as in vivo in nude mice as ascites. One ml of crude culture supernatant can neutralize 2000 units of MuIFN gamma antiviral activity, whereas, the ascitic fluids are 1,000 times more potent. Characterization studies as to the potential of the MAb to neutralize various biological activities of lymphokine preparations thought to be mediated by MuIFN gamma, have been initiated.
- Medicine and Medical Research