Development of Vaccines to Prevent Wound Infections due to Anaerobic Bacteria
Annual rept. no. 7, 1 Sep 1979-31 Aug 1980
PETER BENT BRIGHAM HOSPITAL BOSTON MA
Pagination or Media Count:
The amount of capsular antigen produced by Bacteroides fragilis appears to significantly decrease with in vitro passage on blood agar plates of strain 23745. This decrease in the quantity of isolatable capsule is associated with the emergence of a small transparent colony type variant SCT. The small colony type strain also has a glycogen-like material associated with its outer membrane. This glycogen is found intracellulary, but is not associated with the outer membrane on the mucoid colony variant which predominates after animal passage, the large colony type LCT. There is no concomitant alteration in outer membrane proteins or lipopolysaccharides associated with this colonial transformation. A series of experiments were designed in the model of intraabdominal sepsis to determine the cellular mechanisms of immunity induced by the capsular antigen to both the bacteremic and abscess phase of this disease. These studies which are presented in the second section of this report conclude that both cellular and humoral limbs of the immune system play important roles in protection against B fragilis infection.
- Medicine and Medical Research