The Response of Rats to Cutaneous Dosing with Trichothecene Mycotoxins,
MATERIALS RESEARCH LABS ASCOT VALE (AUSTRALIA)
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T-2 toxin and diacetoxyscirpenol DAS formed deep necrotic ulcers on rat skin at doses of 2 micrograms and 6 micrograms respectively. Verrucarin A and Roridin A were relatively ineffective at producing skin lesions. The percutaneous LD50 of T-2 or DAS was found to be well in excess of 10 mgkg. DMSO had no marked effect in increasing wound severity or systematic toxicity as opposed to ethyl acetate as the application vehicle. No synergism between T-2 and DAS was found. Histological examination of skin lesions revealed the typical inflammatory reaction that is also caused by thermal burns or other necrotising agents. Thin layer chromatography of the toxins is described, as a method to check purity and stability.