Distribution and Characterization of Antigens Found in Subcellular Fractions of African Trypanosomes.
Annual rept. no. 1, 1 Mar-20 Aug 79,
MIAMI UNIV FLA DEPT OF MICROBIOLOGY
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Work was aimed at characterizing the subcellular distribution and nature of antigens found in the African trypanosome, Trypanosoma rhodesiense, with an eventual aim of assessing such antigens for their potential in immune prophylaxis. Attempts have been made to identify enzymes that could be used as markers to characterize the various subcellular fractions obtained after isopycnic gradient centrifugation. Differential centrifugation has established the gross distribution of various enzyme activities between particle fractions and a soluble cell sap fraction. A surface membrane ATPase has been tentatively identified which shows no NaK stimulation or inhibition in the presence of oubain. Results using several phosphates substrates suggest the presence of at least two distinct latent phosphatases, differing in subcellular distribution. One is an acid phosphatase, principally active against beta-glycerophosphate and localized in the smaller subcellular particles. The other having a slightly higher pH optimum is more evenly distributed between small and large particles and principally active against beta-glycerophosphate. An authentic glucose-6-phosphatase may be present but it is not associated with microsomal elements as in other cells.
- Medicine and Medical Research