Enzymatic Conversion of Red Cells for Transfusion.
Annual rept. 1 Mar-30 Nov 81,
NEW YORK BLOOD CENTER N Y
Pagination or Media Count:
We have developed a procedure for the isolation and purification of coffee bean alpha-galactosidase using both classical and affinity chromatographic methods. On the order of 5,000 units of crude enzyme can be extracted from 1 kg of green beans. Initial studies indicated that at least 70 of the crude material is recoverable in purified form essentially devoid of contaminating exoglycosidase and protease activities. We have been searching in both the animal and microbial kingdoms for the appropriate alpha N-acetylgalactosaminidase A-zyme needed to convert group A cells to group 0. With regard to the former, we are currently working with an enzyme present in the limpet Patella vulgata. This enzyme has been reported to destroy A blood group activity present in blood group substances when used at pH 4. Studies are reported on the in Vivo Survival Properties of Enzymatically Converted Type B Human Erythrocytes.