Human Platelet Senescence Study.
Final summary rept. Jul 68-Sep 79,
NEW YORK UNIV NY SCHOOL OF MEDICINE
Pagination or Media Count:
A technique was developed for the identification of platelet membrane antigens. This involves ager electrophoresis in one direction, followed by rotation of the slide 90 deg and electrophoresis into agar containing anti-platelet membrane antibody. Nineteen different platelet membrane antigens immunoprecipitates can be detected. These include Albumin, fibrinogen and an antigen absent in platelets from patients with Glanzmanns thromobasthenia. The relative cell surface antigens can be detected by adsorption studies with intact platelets. The presence of carbohydrate can be detected with suitable lectins, such as concanavalin A used an intermediate spacer gel. Colchicine, an antimicortubule agent stimulates dephosphorylation of phosphorylated platelet cell sap. This effect is inhibited by D20, a microtubule stabilizing agent. Similar results are obtained with cyclic AMP. It is suggested that colchicine operates via activating an adenylate cyclase, which is inhibited by microtubules.
- Medicine and Medical Research