Evaluation of the Stability of Hemoglobin in Preparation and Storage by Electron Spin Resonance.
Annual summary rept. no. 2, Sep 81-Sep 82,
CALIFORNIA UNIV BERKELEY
Pagination or Media Count:
A series of hemoglobin samples were received from LAIR. They were stored in two types of containers at 4 C and one set was frozen at dry ice temperatures. These samples were analyzed using ESR for metHb and hemichrome content as a function of storage time. The 4 C samples stored in glass bottles had a very uniform increase in metHb which was linear in time over the five months they were studied. The frozen samples had no changes in their metHb with time once they were frozen. The 4 C sample stored in a blood-pack had a noticably slower increase in metHb with time. The hemichrome content of only a special sample was analyzed by double integration of it ESR spectrum and we found its metHb to be 4.5 0.4 gdL and its hemichrome to be 5 1 gdL. It is proposed that the two different types of hemichromes are both in chemical equilibrium with the metHb in the samples. The two equilibrium constants appear to vary with the nature of the samples.
- Medicine and Medical Research