Accession Number:

ADA139007

Title:

Pathogenesis of Salmonellosis: Salmonella Exotoxins

Descriptive Note:

Annual progress rept. no. 4, Aug 1980-Dec 1981, Final rept. Dec 1977-Dec 1981

Corporate Author:

TEXAS UNIV MEDICAL BRANCH AT GALVESTON

Personal Author(s):

Report Date:

1982-03-08

Pagination or Media Count:

22.0

Abstract:

We are now using several rapid, in vitro methods to quantitate Salmonella toxin contained in culture filtrates and sonicated cell preparations. Included among these newer techniques are the Chinese hamster ovary CHO cell alongation assay, the CHO floating cell assay, the pigeon erythrocyte lysate assay, two new agar plate techniques for detecting toxin from Salmonella colonies, and an enzyme linked immunosorbent assay. Detailed explanations of each assay are presented later in this section. The behavior of Salmonella toxin in each of these assays is indistinguishable from that of purified cholera toxin, and we have adopted the policy of expressing Salmonella toxin values in terms of chloera toxin equivalents ngml based on direct comparison in each assay with a set of cholera toxin standard solutions. Antigenic and biologic similarities of Salmonella toxin to chloera toxin have been reported previously.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE