The Effects of Hydrazines and Related Compounds on Calcium Calmodulin Regulated Synaptic Processes
Final scientific rept. 1 Jun 1982-1 Jul 1983
YALE UNIV NEW HAVEN CT DEPT OF NEUROLOGY
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This research effort studied the effects of hydrazines and organophosphates on various synaptic processes. The cyclic nucleotide related portions of this study included an examination of the effects of hydrazines upon synaptic membrane adenylate cyclase, cyclic nucleotides and any dynamic membrane events which might be related to the above processes. We were able to show distinct effects of hydrazines upon both catalytic moiety and catalytic moiety-G unit regulated adenylate cyclase. The calcium calmodulin kinase studies in this project investigated the effects of hydrazines and organophosphates on isolated membrane fractions and intact synaptosome preparations. We found that hydrazines and organophosphates have effects on the incorporation of 32 p-phosphate into several specific synaptic proteins and attempted to probe the molecular mechanism mediating the effects of these compounds. These studies provide an insight into the toxic effects of hydrazines and organophosphates on neuronal tissue and may help develop effective methods to prevent and treat these effects in man.
- Anatomy and Physiology