Electron Microscopy of Intracellular Protozoa.
Rept. no. 3 (Annual),
CASE WESTERN RESERVE UNIV CLEVELAND OHIO INST OF PATHOLOGY
Pagination or Media Count:
We studied the interactions between macrophage-like cells P388D and Leishmania braziliensis in vitro under various conditions by light and electron microscopy. When P388D and L. braziliensis were incubated together for two minutes, 90 of the promastigotes were attached to P388D by the tip of the flagella. The P388D did not form pseudopods or aggregated microfilaments around the inserted flagellum. After 5 minute incubation period, the parasites attached to the P388D did not show preferred orientation. At this time, numerous pseudopods and aggregated microfilaments of the P388D were seen around the invading parasites. Studies on the structural alteration of erythocyte membrane during malarial parasite invasion and during subsequent intraerythrocytic development were performed. Erythrocyte entry by malarial merozoites caused structural alteration of the erythocyte membrane. In addition, we studied by electron microscopy the effects of monoclonals on epimastigotes and metacyclic stages of Trypanosoma cruzi. The metacyclic stages of T. cruzi were covered with a thick surface coat when they were incubated with monoclonal B 25. Adhesion between the surface coat of these parasites produced agglutination of the parasites. The agglutination caused by the monoclonal is specific to this stage. No agglutination of the parasites took place between the parasites when incubated with anti- P. knowlesi antibody.
- Medicine and Medical Research