Electron Microscopy of Intracellular Protozoa.
Rept. no. 1 (Annual),
CASE WESTERN RESERVE UNIV CLEVELAND OHIO INST OF PATHOLOGY
Pagination or Media Count:
We studied a inhibitory effects of immune monkey serum on synchronized P. falciparum cultures and b antibody-dependent phagocytosis of Trypanosoma rhodesiense by murine macrophages. The effects of heat-inactivated immune monkey serum on the growth of intra-erythrocytic Plasmodium falciparum were light microscopically indistinguishable from those cultures in normal serum. However, immune serum reduced by 90 the number of erythrocytes containing newly invaded rings. Studies on phagocytosis of Trypanosoma rhodesiense by macrophages showed that macrophages can bind and ingest the parasites in the presence of specific antibody. The complement system participates in the association of macrophages and T. rhodesiense, although antibody-dependent binding of trypanosomes to macrophages was also observed in the presence of antiserum depleted of complement by heating at 53 C for 90 minutes. However, when lower, less effective concentrations of antiserum were utilized, the addition of complement profoundly amplified the association of trypanosomes and macrophages.
- Medicine and Medical Research