Electron Microscopy of Intracellular Protozoa.
Annual rept. no. 1, 1 Jan-15 Aug 79,
CASE-WESTERN RESERVE UNIV CLEVELAND OH
Pagination or Media Count:
The effects of immune serum on P. falciparum-infected erythrocytes grown in vitro were investigated by electron microscopy. When immune serum was added to partially synchronized cultures of P. falciparum, parasites matured normally during the first day from ring forms to trophozoites and schizonts. In contrast, the number of new rings detected two days following reinvasion was reduced by 90, compared with cultures containing normal serum. Clusters of merozoites were seen in cultures grown with immune serum, but rarely in cultures with normal serum. Electron microscopy showed many extracellular merozoites in the culture with immune serum. The effects of immune serum on sporozoites of P. bergjei and P. knowlesi were also studied. Sporozoites incubated in normal serum interact with macrophage and actively penetrate into the macrophages. On the other hand, sporozoites incubated in immune serum showed a distinct surface coat and were phagositized by the macrophages.
- Medicine and Medical Research