A Competitive Displacement Assay to Detect Saxitoxin and Tetrodotoxin
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Pagination or Media Count:
An assay is described which detects saxitoxin STX and tetrodotoxin TTX by their competitive displacement of 3H-saxitoxin from its receptor in rat brain membranes. The assay has a sensitivity of 0.15 ng STXm1 and 0.8 ng TTXm1 for buffer samples. The assay was also applied to detection of these toxins in unextracted human plasma and found to have a sensitivity of 0.5 ng STXm1 and 0. 6 ng TTXm1. The competitive displacement assay appears to be the most sensitive procedure yet for detection of STX and TTX.