Evaluation Studies of the DEN-2/S-1 Vaccine.
Final rept. 1 Apr 80-3 Nov 82, Annual rept. Aug 81-Nov 82,
PUERTO RICO UNIV SAN JUAN
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The purpose of this study was to determine how much dengue 2 and dengue 4 viruses were required to infect rhesus monkeys. To this effect, groups of five dengue antibody free animals were inoculated subcutaneously with serial 10 fold dilutions of dengue 2 PR159 and dengue 4 H241 viruses. Virus titrations were carried out simultaneously by direct plaque assay in LLC-MK2 cells, by mosquito inoculation using both Aedes aegypti and Toxorynchites amboiensis and in the monkeys. Blood samples for serology were taken at 30 and 60 days post infection. Neutralization tests revealed that 9.5 mosquito infectious do se 50 MID50 of dengue 2 and 22 MID50 of dengue 4 were required to infect 50 of the monkeys. The data suggest that in receipients challenges with wild type virus, a minimum of 100 MID50s should be used as the virus challenge dose.
- Medicine and Medical Research