Investigation of Immunoregulatory Alphaglobulin (IRA) in Shock and Trauma.
Annual progress rept. 1 Jul 78-30 Jun 79,
PETER BENT BRIGHAM HOSPITAL BOSTON MA
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The major accomplishment of the past year of research was the characterization of the substance chiefly responsible for the immunosuppressive activity appearing in the serum of patients following major trauma and burns. Pooled serum from such patients was subjected to DEAE cellulose chromatography and further fractionated by gel filtration on G25 Sephadex. A low molecular weight fraction was found to contain the majority of the suppressive activity as determined by its ability to suppress PHA stimulatioln of normal human peripheral blood lymphocytes in tissue culture. It was also demonstrated that, at a dose of approximately 5 mg per animal, this low molecular weight fraction suppressed the plaque forming cell response to sheep erythrocytes in mice by more than 50. The low molecular weight suppressive fraction was further fractionated by preparative high-voltage electrophoresis in distilled water and acetic acid. Individuals ninhydrin positive moieties were eluted from filter paper and recovered by lyophilization. These fractions were tested for suppressive activity in vitro and the majority of the activity was found in a highly basic fraction, fraction 11. This highly basic molecular species was not recovered from similarly processed serum from patients who had undergone minor surgical trauma or from normal volunteers.
- Medicine and Medical Research