An Investigation of Membrane-Encapsulated Trypanocides.
Annual rept. 1 Jul 78-15 Jan 79,
WASHINGTON UNIV SEATTLE
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A general method of loading 111 In 3 to liposomes by a mobile ionophore, 8-hydroxyquinoline, has been developed to encapsulate a high specific radio-activity of 111 In 3 in liposomes for investigating the fate of liposomes in tissues. Liposomes prepared by 8-hydroxyquinoline 111 In 3 loading procedure have a similar tissue distribution as liposomes prepared by standard methods of encapsulation. In the study of the prolongation of drug carriers in blood circulation our results suggest that unilamellar liposomes of about 350 deg A in diameter can serve as vehicles to prolong an encapsulated drug in circulation for extended periods of time. The routes of the administration of this type of liposomes do not seem to alter the blood clearance time of the small unilamellar liposomes. However, the extent of hepatic uptake of liposomes seem to decrease, if liposomes are given by intraperitoneal injection instead of intraveneous injection. Similar results have also been observed in liposomes of about 650 deg A in diameter, except that an extensive uptake of liposomes by skin has been found within 2 hrs. after the intraperitoneal injection of liposomes. This suggests that peritoneal cavity. Furthermore, the complex of ethylene-diaminetetraacetic and 111 In 3 may be a better marker than 99mTc04- in future studies of lipid-protein vesicles of serum.
- Medicine and Medical Research