Detection of T-2 Toxin by an Improved Radioimmunoassay
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Pagination or Media Count:
T-2 toxin in serum, urine, and saline was analyzed by a modified radioimmunoassay procedure. The specimens were added directly to the assay tubes without extraction steps. The reaction between antibody and ligands was optimal at 1-h. Albumin-coated charcoal was used to separate bound from free radioactivity. Quenching, which occurred with hemolyzed specimens, was corrected by a wet oxidation process with 60 perchloric acid and 30 hydrogen peroxide. The shorter incubation times resulted in an assay that takes less than 6 h to complete. The average affinity constant of the antibody K sub m was 1.75 x 10 expn 10 litersmol. The sensitivity was 1 ng per assay or 10 ngml. Among the other trichothecenes tested, only H-T-2 cross-reacted significantly 10.3.