Accession Number:

ADA133843

Title:

Detection of T-2 Toxin by an Improved Radioimmunoassay

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

1983-02-01

Pagination or Media Count:

4.0

Abstract:

T-2 toxin in serum, urine, and saline was analyzed by a modified radioimmunoassay procedure. The specimens were added directly to the assay tubes without extraction steps. The reaction between antibody and ligands was optimal at 1-h. Albumin-coated charcoal was used to separate bound from free radioactivity. Quenching, which occurred with hemolyzed specimens, was corrected by a wet oxidation process with 60 perchloric acid and 30 hydrogen peroxide. The shorter incubation times resulted in an assay that takes less than 6 h to complete. The average affinity constant of the antibody K sub m was 1.75 x 10 expn 10 litersmol. The sensitivity was 1 ng per assay or 10 ngml. Among the other trichothecenes tested, only H-T-2 cross-reacted significantly 10.3.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE