Effect of Radiofrequency Radiation on DNA Duplex Stability and Replication.
Final rept. 30 Apr 81-30 Nov 82,
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Three experimental approaches were used to determine if absorption of continuous wave radiofrequency RF photons affect the stability andor replication of mammalian DNA. Two of the approaches involved experiments with female CD-1 mice, including analyses of RF effects on sister chromatid exchanges SCE in bone marrow as one index of DNA stability and RF effects on replication of the animals marrow and spleen DNA. The third experimental approach consisted of tests to determine if RF exposure causes partial denaturation of double-stranded DNA, monitored in this study by susceptibility of the polymer to hydrolysis by single-strand-specific Sl nuclease. The substrate DNA used for this cell-free assay was isolated from Chinese hamster ovary cell cultures labeled with radioactive deoxynucleosides. Irradiated subjects were exposed to incident RF field densities adjusted to be equivalent to absorbed doses in mice of 4 Wkg at each of three test frequencies 400, 800, and 1200 MHz. The results revealed no reduction in the level of DNA synthesis in either the spleen or the bone marrow of animals exposed to any of the three test frequencies, also no increase in the number of SCE. However, a slight, but consistent, increase in the nuclease susceptibility of isolated DNA appeared to be a result of RFR exposure.